Flat-screen televisions throughout the hospital project an image of the State's leader giving a speech calling for greater conformity.Įventually, a handsome man (again, by the contemporary viewer's standards) named Walter Smith arrives to take the crying, despondent Janet Tyler into exile to a village of her "own kind", where her "ugliness" will not trouble the State. Distraught by the failure of the procedure, Janet runs through the hospital as what is considered normal in this alternate society "State" is revealed. The camera pulls back to reveal that, by the contemporary viewer's standards, Janet is beautiful by those same standards, the doctor, nurses and other people in the hospital are ugly, with drooping features, large, thick brows, sunken-in eyes, swollen and twisted lips, and wrinkled noses with extremely large nostrils, almost like pigs' snouts. The doctor removes the bandages, and announces that the procedure has failed, and her face has undergone no change. The nurse warns him not to continue in that vein, as it is considered treason. The doctor becomes displeased and questions why Janet or anyone must be judged on their outer beauty. The nurse expresses concern for the doctor and admits she still is uneasy about Janet's appearance. As he prepares, the doctor develops great empathy for his patient. Unable to bear the bandages any longer, Janet pleads with the doctor and eventually convinces him to remove them early. The outcome of the procedure cannot be known until the bandages are removed. Her face is described as a "pitiful twisted lump of flesh" by the nurses and doctor, whose own faces are always in shadows or off-camera. She is shown with her head completely bandaged so that her face cannot be seen. Janet Tyler has undergone her eleventh treatment (the maximum number legally allowed) in an attempt to look normal. This happens to be the Twilight Zone, and Miss Janet Tyler, with you, is about to enter it. Keeping in mind of course that we are not to be surprised by what we see, because this isn't just a hospital, and this patient 307 is not just a woman. In a moment we will go back into this room, and also in a moment we will look under those bandages. A universe whose dimensions are the size, thickness, length of the swath of bandages that cover her face. In conclusion, we found that SameSpots represents improvements both in reproducibility and objectivity in relation to previous generations of 2-DE analysis software.Suspended in time and space for a moment, your introduction to Miss Janet Tyler, who lives in a very private world of darkness. In terms of statistical power, the less-experienced user achieved the better results, but no discernible difference was detected in multivariate comparisons between the users. Evaluations of the software-induced variance in relation to other sources of variance, as well as the subjectivity through comparison of analyses performed by an expert user and a novice lab-user, were performed. We evaluated the performance of SameSpots, representing a new generation of 2-DE image analysis software, using both DIGE and traditional single-stain 2-DE approaches. Eye of the beholder 3 intro manual#Limitations in image alignment and spot detection of previous generations of 2-DE analysis software have demanded considerable user-intervention and manual editing, resulting in introduction of a large degree of subjectivity and software-induced variance. However, as the confounding gel-to-gel variation improves, the variance arising from the subsequent image analysis becomes more prominent. Over the past decade, a range of technological leaps have reduced the overall variance of 2-DE, thus turning the technology into a robust platform for quantitative intact proteomics. Historically, the use of two-dimensional electrophoresis (2-DE) in quantitative proteomics has been hampered by significant technical variance.
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